Tungsten in PDB 6eth: The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate

All present enzymatic activity of The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate:
2.10.1.1; 2.7.7.75;

The structure of The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate, PDB code: 6eth was solved by J.Krausze, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.58 / 1.64
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	65.500, 123.470, 133.600, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 20

The structure of The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Tungsten atom in the The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate (pdb code 6eth). This binding sites where shown within 5.0 Angstroms radius around Tungsten atom.
In total 2 binding sites of Tungsten where determined in the The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate, PDB code: 6eth:
Jump to Tungsten binding site number: 1; 2;

Tungsten binding site 1 out of 2 in the The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate


Mono view


Stereo pair view

A full contact list of Tungsten with other atoms in the W binding site number 1 of The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate within 5.0Å range: probe atom residue distance (Å) B Occ A:W503 b:47.0 occ:0.70 W A:WO4503 0.0 47.0 0.7 O1 A:WO4503 1.2 56.1 0.3 O1 A:WO4503 1.8 46.6 0.7 O4 A:WO4503 1.8 51.4 0.7 O3 A:WO4503 1.8 47.8 0.7 O2 A:WO4503 1.8 52.1 0.7 W A:WO4503 1.8 55.8 0.3 O4 A:WO4503 2.4 56.3 0.3 O2 A:WO4503 2.6 55.5 0.3 HH12 A:ARG369 2.8 54.4 0.0 HA A:PRO295 3.2 38.8 0.0 O A:HOH793 3.3 71.2 1.0 H A:GLY296 3.4 47.9 0.0 HH22 A:ARG369 3.4 48.7 0.0 HG A:SER400 3.4 42.3 0.0 O3 A:WO4503 3.5 56.1 0.3 HB3 A:SER400 3.6 37.5 0.0 NH1 A:ARG369 3.8 54.9 1.0 HG3 A:LYS294 3.9 44.9 0.0 H A:LYS297 4.0 48.0 0.0 CA A:PRO295 4.1 38.2 1.0 OG A:SER400 4.2 41.7 1.0 N A:GLY296 4.2 47.8 1.0 HB3 A:PRO295 4.3 38.5 0.0 NH2 A:ARG369 4.3 49.2 1.0 HG A:SER328 4.3 34.5 0.0 HB2 A:PRO295 4.3 37.7 0.0 HA A:LYS294 4.3 38.7 0.0 CB A:SER400 4.4 37.1 1.0 O A:HOH776 4.4 41.6 1.0 CB A:PRO295 4.4 38.0 1.0 HH11 A:ARG369 4.5 54.8 0.0 HZ1 A:LYS294 4.5 89.0 0.0 O A:HOH691 4.5 39.9 1.0 CZ A:ARG369 4.6 63.9 1.0 O A:HOH607 4.6 39.7 0.7 C A:PRO295 4.7 47.1 1.0 OG A:SER328 4.8 34.1 1.0 HB2 A:SER400 4.8 36.6 0.0 H A:SER400 4.9 34.6 0.0 CG A:LYS294 4.9 44.9 1.0 HZ2 A:LYS294 5.0 90.0 0.0

Tungsten binding site 2 out of 2 in the The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate


Mono view


Stereo pair view

A full contact list of Tungsten with other atoms in the W binding site number 2 of The Structure of the Mo-Insertase Domain CNX1E From Arabidopsis Thaliana in Complex with Amp and Tungstate within 5.0Å range: probe atom residue distance (Å) B Occ A:W503 b:55.8 occ:0.30 W A:WO4503 0.0 55.8 0.3 O1 A:WO4503 1.6 46.6 0.7 O1 A:WO4503 1.8 56.1 0.3 O4 A:WO4503 1.8 56.3 0.3 O3 A:WO4503 1.8 56.1 0.3 O2 A:WO4503 1.8 55.5 0.3 W A:WO4503 1.8 47.0 0.7 O2 A:WO4503 2.0 52.1 0.7 O3 A:WO4503 2.5 47.8 0.7 HZ1 A:LYS294 3.1 89.0 0.0 O A:HOH607 3.1 39.7 0.7 HG A:SER400 3.1 42.3 0.0 HH22 A:ARG369 3.2 48.7 0.0 O4 A:WO4503 3.6 51.4 0.7 HB3 A:SER400 3.7 37.5 0.0 O A:HOH691 3.7 39.9 1.0 HG3 A:LYS294 3.7 44.9 0.0 HH12 A:ARG369 3.9 54.4 0.0 H A:SER400 3.9 34.6 0.0 HZ2 A:LYS294 3.9 90.0 0.0 NZ A:LYS294 3.9 89.7 1.0 OG A:SER400 4.1 41.7 1.0 HA A:PRO295 4.1 38.8 0.0 O A:HOH850 4.1 87.3 1.0 NH2 A:ARG369 4.2 49.2 1.0 CB A:SER400 4.4 37.1 1.0 HZ3 A:LYS294 4.5 90.3 0.0 HB3 A:PRO295 4.5 38.5 0.0 O A:HOH793 4.6 71.2 1.0 CG A:LYS294 4.7 44.9 1.0 NH1 A:ARG369 4.7 54.9 1.0 N A:SER400 4.8 34.0 1.0 HH21 A:ARG369 4.8 50.0 0.0 HE3 A:LYS294 4.8 74.2 0.0 HG2 A:LYS294 4.8 44.4 0.0 HA A:MET399 4.9 38.9 0.0 CE A:LYS294 4.9 75.0 1.0 CZ A:ARG369 5.0 63.9 1.0 H A:GLY296 5.0 47.9 0.0

J.Krausze, T.W.Hercher, D.Zwerschke, M.L.Kirk, W.Blankenfeldt, R.R.Mendel, T.Kruse. The Functional Principle of Eukaryotic Molybdenum Insertases. Biochem. J. V. 475 1739 2018.
ISSN: ESSN 1470-8728
PubMed: 29717023
DOI: 10.1042/BCJ20170935