Tungsten in PDB 2ush: 5'-Nucleotidase From E. Coli

All present enzymatic activity of 5'-Nucleotidase From E. Coli:
3.1.3.5;

The structure of 5'-Nucleotidase From E. Coli, PDB code: 2ush was solved by T.Knofel, N.Strater, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.22
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	93.210, 116.290, 132.770, 90.00, 90.00, 90.00
R / Rfree (%)	19.8 / 25.4

The structure of 5'-Nucleotidase From E. Coli also contains other interesting chemical elements:

Zinc

(Zn)

9 atoms

The binding sites of Tungsten atom in the 5'-Nucleotidase From E. Coli (pdb code 2ush). This binding sites where shown within 5.0 Angstroms radius around Tungsten atom.
In total 4 binding sites of Tungsten where determined in the 5'-Nucleotidase From E. Coli, PDB code: 2ush:
Jump to Tungsten binding site number: 1; 2; 3; 4;

Tungsten binding site 1 out of 4 in the 5'-Nucleotidase From E. Coli


Mono view


Stereo pair view

A full contact list of Tungsten with other atoms in the W binding site number 1 of 5'-Nucleotidase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:W702 b:55.1 occ:0.50 W A:WO4702 0.0 55.1 0.5 O1 A:WO4702 1.8 48.9 0.5 O4 A:WO4702 1.8 58.3 0.5 O3 A:WO4702 1.8 59.0 0.5 O2 A:WO4702 1.8 53.0 0.5 O2 A:WO4703 2.2 38.0 0.5 O4 B:WO4704 3.2 59.4 0.5 W A:WO4703 3.4 36.2 0.5 O1 A:WO4703 3.5 15.2 0.5 O1 B:WO4704 3.6 60.5 0.5 W B:WO4704 4.0 74.7 0.5 NH2 B:ARG410 4.2 51.3 1.0 NH2 A:ARG375 4.3 52.0 1.0 O3 A:WO4703 4.3 36.1 0.5 O4 A:WO4703 4.8 16.8 0.5 NH1 A:ARG379 4.8 48.3 1.0 O3 B:WO4704 4.9 54.9 0.5

Tungsten binding site 2 out of 4 in the 5'-Nucleotidase From E. Coli


Mono view


Stereo pair view

A full contact list of Tungsten with other atoms in the W binding site number 2 of 5'-Nucleotidase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ A:W703 b:36.2 occ:0.50 W A:WO4703 0.0 36.2 0.5 O4 A:WO4703 1.8 16.8 0.5 O3 A:WO4703 1.8 36.1 0.5 O2 A:WO4703 1.8 38.0 0.5 O1 A:WO4703 1.8 15.2 0.5 O3 A:WO4702 2.1 59.0 0.5 W A:WO4702 3.4 55.1 0.5 O4 A:WO4702 3.8 58.3 0.5 ZN A:ZN709 3.8 31.9 0.5 O4 B:WO4704 3.8 59.4 0.5 O3 B:WO4704 3.8 54.9 0.5 O2 A:WO4702 4.0 53.0 0.5 NH1 A:ARG375 4.2 56.3 1.0 NH2 A:ARG379 4.3 48.5 1.0 NH2 B:ARG375 4.3 80.8 1.0 W B:WO4704 4.3 74.7 0.5 NH1 A:ARG410 4.4 30.3 1.0 NH2 A:ARG410 4.4 38.8 1.0 NH2 A:ARG375 4.5 52.0 1.0 O1 B:WO4704 4.7 60.5 0.5 CZ B:ARG375 4.7 78.3 1.0 NH1 A:ARG379 4.8 48.3 1.0 CZ A:ARG375 4.9 60.4 1.0 O1 A:WO4702 4.9 48.9 0.5 CZ A:ARG410 4.9 39.6 1.0

Tungsten binding site 3 out of 4 in the 5'-Nucleotidase From E. Coli


Mono view


Stereo pair view

A full contact list of Tungsten with other atoms in the W binding site number 3 of 5'-Nucleotidase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:W701 b:88.4 occ:0.50 W B:WO4701 0.0 88.4 0.5 O2 B:WO4701 1.8 83.6 0.5 O1 B:WO4701 1.8 64.5 0.5 O3 B:WO4701 1.8 68.3 0.5 O4 B:WO4701 1.8 70.5 0.5 O1 B:WO4704 3.7 60.5 0.5 NZ B:LYS322 4.1 46.3 1.0 O2 A:WO4702 4.6 53.0 0.5 NZ A:LYS322 5.0 58.1 1.0

Tungsten binding site 4 out of 4 in the 5'-Nucleotidase From E. Coli


Mono view


Stereo pair view

A full contact list of Tungsten with other atoms in the W binding site number 4 of 5'-Nucleotidase From E. Coli within 5.0Å range: probe atom residue distance (Å) B Occ B:W704 b:74.7 occ:0.50 W B:WO4704 0.0 74.7 0.5 O2 B:WO4704 1.8 53.0 0.5 O4 B:WO4704 1.8 59.4 0.5 O1 B:WO4704 1.8 60.5 0.5 O3 B:WO4704 1.8 54.9 0.5 O2 A:WO4702 2.8 53.0 0.5 O2 A:WO4703 3.0 38.0 0.5 O3 B:WO4701 3.7 68.3 0.5 NH2 B:ARG410 3.9 51.3 1.0 W A:WO4702 4.0 55.1 0.5 O4 A:WO4702 4.1 58.3 0.5 O4 A:WO4703 4.3 16.8 0.5 W A:WO4703 4.3 36.2 0.5 NH2 B:ARG379 4.5 40.6 1.0 NH1 B:ARG410 4.5 42.1 1.0 O3 A:WO4702 4.6 59.0 0.5 CZ B:ARG410 4.7 46.7 1.0

T.Knofel, N.Strater. X-Ray Structure of the Escherichia Coli Periplasmic 5'-Nucleotidase Containing A Dimetal Catalytic Site. Nat.Struct.Biol. V. 6 448 1999.
ISSN: ISSN 1072-8368
PubMed: 10331872
DOI: 10.1038/8253